End-To-End Solution for Microbial Whole Genome Sequencing​

Fast, high-quality microbial WGS—from sample to analysis

Fast

5 hours for SE100 & 12 hours for PE150 sequencing.

Cost Effective

Sequencing cost from $27 per sample.

Flexible

80M reads per flow cell (4 to 24 samples), 2 flow cells per instrument.

Microbial Whole Genome Sequencing Overview

Microbial whole genome sequencing (WGS) impacts a wide-range of application areas including, but not limited to food safety and environmental research, antimicrobial resistance genes and mechanisms, and vaccine development. It’s a method to sequence the entire genome of a microbe (bacteria, fungus, or other microbial genomes). Compared to traditional approaches, such as PCR, microbial WGS provides more comprehensive information.​

Fast, High-Quality, and Affordable Microbial WGS with DNBSEQ-G99

Complete Genomics provides end-to-end microbial whole genome sequencing for culture isolates from sample extraction through analysis. After extraction, users can prepare sequencing libraries through either manual or various throughput automation workflows. Once the sequencing libraries are constructed, they can be sequenced on a DNBSEQ-G99 benchtop sequencer that provides fast, high-quality, flexible, and cost-effective performance.

  • Fast: 5 hours for SE100, 12 hours for PE150 sequencing​
  • High quality: 85% > Q40​
  • Flexible: 80M reads (4-24 samples) per flow cell, two independent flow cells per instrument​
  • Cost effective: sequencing cost from $27 per sample (based on 24 samples per flow cell)​

After sequencing, users have the option of either analyzing their data with the Platform of Microorganisms Fast Identification and Assembly Evolution (PFI Pro) or their own solution.​

DNBSEQ-G99-Complete-Genomics-Front

Microbial WGS Workflow

Library Prep

DNBelab-D4 (4 samples)
SP-100 (8-16 samples)
SP-Smart8 (1-48 samples)
DNBSEQ Fast FS Library Prep
DNBSEQ OneStep Make DNB Reagent Kit (dual barcode)

Sequencing

DNBSEQ-G99 Genetic Sequencer

SE100 or PE150 Read Length
4-24 Samples / Flow Cell

Analysis

Platform of Microorganisms Fast Identification and Assembly Evolution (PFI Pro)

Ordering Information

CategoryProductCat. No.
Sample Prep​NE32 Automated Nucleic Acid Extractor​950-000020-00​
MGIEasy Microbiome DNA Extraction Kit​1000027955​
Library Prep ​DNBSEQ Fast FS Library Prep Set V2.0 (16 RXN)​940-001511-00​
DNBSEQ Fast FS Library Prep Set V2.0 (96 RXN)​940-001509-00​
DNBSEQ OneStep Make DNB Reagent Kit V4.0 (Dual Barcode), 4 RXN​940-001750-00​
AutomationDNBelab-D4 Digital Sample Preparation System​900-000822-00
DNBelab-D4RS Fast FS DNA Library Prep Set 2.0 (16-test)​​940-001198-00​
MGISP-100 RS Automated Sample Preparation System​900-000206-00​
MGISP-Smart 8RS Sample Preparation System Configuration 4​900-000503-00​
SequencingGenetic Sequencer DNBSEQ-G99900-000713-00​
DNBSEQ-G99 High-throughput Sequencing Reagent Set V2.0 (App-D FCL SE100)​940-001874-00​
DNBSEQ-G99 High-throughput Sequencing Reagent Set V2.0 (App-D FCL PE150)​940-001871-00​
AnalysisPlatform of Microorganisms Fast Identification and Assembly Evolution (PFI Pro)​900-000399-00​

A Complete Solution for Every Step of Your Workflow