Stereo-seq Transcriptomics FF v1.3
Single-Cell Resolution Spatial Transcriptomics for FF Tissues
From Tissue Architecture to Drug Discovery Insights
Unprecedented Spatial Mapping of Biology in Fresh-Frozen Tissues
Stereo-seq transcriptomics FF integrates single-cell profiling with spatial precision to reveal complex molecular landscapes underlying biology and disease. Stereo-seq leverages the preserved RNA integrity in Fresh-Frozen (FF) tissues from diverse sample types to generate high-resolution gene expression maps. Its unmatched subcellular resolution and large capture area (up to 13 cm × 13 cm) enable spatially resolved single-cell analysis and detailed mapping of cell-to-cell interactions. This makes Stereo-seq ideal for large-scale tissue atlas studies, capturing broad tissue sections in unprecedented detail while supporting multi-scale transcriptomic analysis— from tissue architecture to molecular signatures.
Multi-Omics Flexibility
Unlock unbiased whole-transcriptome discovery and integrated multi-omics capabilities.
Higher Gene Counts
Enhanced capture efficiency, delivering more gene counts per cell for comprehensive transcriptomic insights.
Species-Agnostic
Supports diverse tissue types, species, and low-RIN precious samples.
Unlock Spatial Biology Insights From Sample to Discovery
This end-to-end workflow combines high-resolution spatial transcriptomics with Stereo-seq, precise tissue imaging with Go Optical, robust library preparation and conversion powered by DNBSEQ™ technology, and high-throughput sequencing on the DNBSEQ-T7 sequencer. Paired with intuitive data visualization in StereoMap, it enables exploration of spatial gene expression with unprecedented scale and precision.
Applications
Stereo-seq transcriptomics FF solution helps elucidate spatial information and identify key developmental points, thereby accelerating research in neuroscience, oncology, immunology, and developmental biology. This advancement significantly drives the discovery of novel biomarkers and enhances our understanding of disease progression and potential therapeutic targets.
Stereo-seq Revealed a Potential Therapeutic Target
Stereo-seq transcriptomics FF solution identified transcriptional alterations in neuronal clusters spatially associated with amyloid-beta plaques in a 5xFAD mouse model, revealing ZNF460 as a potential therapeutic target.
The Stereo-seq transcriptomics FF solution’s nanoscale resolution and whole-transcriptome coverage enabled the precise mapping of gene expression changes around plaques, highlighting disruptions in laminar cortical structure and cell-cell interactions during Alzheimer’s disease (AD) progression (Zhang et al., 2023).
Stereo-seq Enabled Mapping Tumor Interactions
Stereo-seq transcriptomics FF solution revealed tertiary lymphoid structures containing CXCL13+ cancer-associated fibroblasts, stem-like CXCL13+CD8+ T-cells, and B/T follicular helper cells.
The spatial precision of the Stereo-seq transcriptomics FF solution showed dynamic interactions with tumor cell aggregates, where plasma cells adjacent to Epstein-Barr virus-infected malignant cells promoted apoptosis via antibody-dependent mechanisms (Liu et al., 2024).
Stereo-seq Uncovered Cellular Diversity in Rat Brain
Stereo-seq transcriptomics FF solution combined with DNBSEQTM sequencing profiled over 1.5 million cells in the rat brain, revealing the nanoscale spatial organization of transcriptionally distinct regions. The integration of ultra-high-resolution Stereo-seq with advanced clustering revealed 15 distinct spatial gene expression clusters, highlighting functional and structural domains within the brain.
This workflow demonstrated how the Stereo-seq FF transcriptomic solution revealed intricate spatial heterogeneity and functional specialization within brain tissue by mapping the differential expression of myelination genes, such as Mbp, Ppl1, and Ugt8, across subpopulations.
Gain Deeper Spatial Insights with StereoMap
StereoMap enables the seamless overlay of gene expression heatmaps onto H&E-stained sections, precise ROI annotation with lasso tools, and dynamic analysis of spatial clusters using adjustable opacity, layers, and segmentation views, providing an interactive platform to explore complex spatial relationships at the single-cell level.
Explore The Power of StereoMap With Our Demo Datasets
Here are a few Mouse tissue data sets, including brain, embryo, eyeball, ovary, and tongue, for you to explore by simply clicking on each image and experiencing interactive spatial data visualization.
Ordering Information
| Category | Product | Cat. No. |
|---|---|---|
| Sample Prep | Stereo-seq Transcriptomics Set for Chip-on-a-slide (0.5cm*0.5cm) V1.3 | 211ST13004-CG |
| Stereo-seq Transcriptomics Set (1cm*2cm) V1.3 | 111ST13122-CG | |
| Stereo-seq Transcriptomics Set (2cm*2cm) V1.3 | 111ST13221-CG | |
| Stereo-seq Transcriptomics Set (2cm*3cm) V1.3 | 111ST13231-CG | |
| Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1 | 211SP11118-CG | |
| Stereo-seq Permeabilization Set (1cm*2cm) V1.1 | 111SP11124-CG | |
| Stereo-seq Permeabilization Set (2cm*2cm) V1.1 | 111SP11224-CG | |
| Stereo-seq Permeabilization Set (2cm*3cm) V1.1 | 111SP11234-CG | |
| Library Prep | Stereo-seq 16 Barcode Library Preparation Kit V1.0 | 111KL160-CG |
| Instruments | STOmics Microscope – Go Optical | 900-001019-00 |
| Genetic Sequencer DNBSEQ-T7 | 900-000698-00 | |
| Sequencing Reagents | DNBSEQ-T7RS Stereo-seq Visualization Reagent Set (FCL PE75) | 940-001889-00 |
| DNBSEQ-G400RS Stereo-seq Visualization Reagent Set (FCL PE75) | 940-001885-00 |