Complete NGS Solutions for Microbiology

Fast, Cost-Effective, Comprehensive

Complete Genomics offers a range of end-to-end NGS solutions for microbiology applications in clinical research, veterinary science, food safety, and environmental testing. 

16S rDNA Sequencing

Sample to results in 39 hours

Sequencing cost as low as $2/sample

The V3-V4 region is highly variable and effective in revealing diverse microbial populations, making it ideal for microbial identification and classification. With PE300 read length and high-speed capabilities, DNBSEQ-G99 is a perfect sequencer for this application.

DNBSEQ-G99

Workflow

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Sample Prep

~2 hrs

Library Prep

~6 hrs

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Sequencing

~12-30 hrs

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Analysis

  • MetaSIS Software
  • MegaBOLT Workstation

~0.5 hr

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Sample Prep

. SP-NE32 System
. MGIEasy Stool Microbiome DNA Extraction Kit II

~2 hrs

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Library Prep

. SP-960 System
. ATOPlex 16S V3V4 rDNA Library Prep Set

~6 hrs

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Sequencing

. DNBSEQ-G99 Sequencer
. App-D FCL PE300 Sequencing Set*

~12-30 hrs

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Analysis

. MetaSIS Software
. MegaBOLT Workstation

~0.5 hr

*Available Q3 2024

Ordering Information

Workflow ​Type​Product name​Catalog No.​
Sample Preparation​Instrument​SP-NE32 Automated Nucleic Acid Extractor950-000020-00​
Reagents​MGIEasy Stool Microbiome DNA Extraction Kit II​940-001247-00 (96RXN)​
Library Preparation​Instrument​SP-960 High-throughput Automated Sample Preparation System (Config. 9)​900-000154-00​
Reagents​ATOPlex 16S V3V4 rDNA Library Preparation Set​940-001261-00 (96RXN)​
940-000725-00 (576RXN)​
ATOPlex E450 Dual Barcode Balanced Library Reagent​940-000637-00​
DNBSEQ Dual Barcode Circularization Kit​940-001310-00 (16RXN)​
940-001309-00 (96RXN)​
Sequencing​Instrument​DNBSEQ-G99 Genetic Sequencer900-000713-00​
Reagents​App-D FCL PE300* High-throughput Sequencing Set​940-001717-00​
Analysis​Software​MetaSIS Software (Metabarcoding Species Identification)​970-000417-00 (unlimited)​
970-000456-00 (96Report)​
Hardware​ MegaBOLT Bioinformatics Analysis Accelerator (Workstation Server)​900-000677-00​

Pathogen Targeted Sequencing

Sample to results in 11 hours

Sequencing cost as low as $25/sample

Pathogen Targeted Sequencing (tNGS), an amplicon-based sequencing, offers fast and cost-effective solutions for rapid screening and identification of common or known pathogens. The tNGS workflow, powered by Complete Genomics’ DNBSEQ-E25 sequencer with the AccuGen* Pathogen kits and software, enables the detection and quantification of 330 common pathogens (178 bacteria, 96 viruses, 56 fungi, and protozoans) and 150 drug-resistant genes from a variety of specimens, including blood, swab, sputum, BAL, urine, CSF, etc.

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DNBSEQ-E25

Workflow

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Sample Prep

  • AccuGen* Pathogen DNA/RNA extraction Kit (50 tests)

~1.5 hrs

Library Prep

  • AccuGen* Pathogen Multiplex-PCR Library Prep Kit (96 tests)

~6 hrs

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Sequencing

~3 hrs

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Analysis

  • AccuGen* Rapid Identification of Pathogen Microorganism Software

~0.5 hr

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Sample Prep

  • AccuGen* Pathogen DNA/RNA extraction Kit (50 tests)

~1.5 hrs

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Library Prep

  • AccuGen* Pathogen Multiplex-PCR Library Prep Kit (96 tests)

~6 hrs

Sequencing

~3 hrs

Analysis

  • AccuGen* Rapid Identification of Pathogen Microorganism Software

~0.5 hr

*Complete Genomics is the sole distributor of AccuGen products, from Health GeneTech (HGT), in the US.

Ordering Information

Workflow ​Type​Product name​Catalog No.​
Sample Preparation​Reagents​AccuGen* Pathogen DNA/RNA Extraction Kit​961-000013-00 (50 tests)​
Library Preparation​Reagents​AccuGen* Pathogen Multiplex-PCR Library Prep Kit​961-000014-00 (96 tests)​
Sequencing​Instrument​DNBSEQ-E25 Genetic Sequencer​900-000703-00​
Reagents​FCL SE100 High-throughput Sequencing Set​940-000894-00​
Analysis​Software​AccuGen Rapid Identification of Pathogen Microorganisms Software​ Pre-installed on DNBSEQ-E25​

Shotgun Metagenomics Sequencing

Sample to results in 11 hours

Sequencing cost as low as $10/sample

Shotgun metagenomic sequencing (mNGS), is a powerful tool for gaining comprehensive insights into the biodiversity and functions of entire microbial communities directly from natural environments such as soil, water, or the human gut. It is espe-cially effective for studying unculturable microorganisms, which traditional methods cannot analyze. Complete Genomics offers automated end-to-end mNGS solutions with various library preparation systems and sequencers tailored to your throughput needs.

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DNBSEQ-G400

Workflow

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Sample Prep

  • SP-NE32 System
  • MGIEasy Nucleic Acid Extraction Kit (Viral DNA+RNA)
  • MGIEasy Microbiome DNA Extraction Kit
  • MGIEasy Stool Microbiome DNA Extraction Kit II

Library Prep

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Sequencing

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Analysis

  • PFI Pro*
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Sample Prep

  • SP-NE32 System
  • MGIEasy Nucleic Acid Extraction Kit (Viral DNA+RNA)
  • MGIEasy Microbiome DNA Extraction Kit
  • MGIEasy Stool Microbiome DNA Extraction Kit II
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Library Prep

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Sequencing

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Analysis

  • PFI Pro*

*Available Q3 2024 **Available Q4 2024

Learn More

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Revolutionizing Personalized Medicine Through Metagenomic Sequencing Strategies

Dr. Maike Seifert, the Head of the Core Lab Unit at the Center for Translational Microbiome Research at the Karolinska Institutet, explores the latest advancements in metagenomic sequencing technologies and their implications for personalized medicine.

Ordering Information

Workflow ​Type​Product name​Catalog No.​
Sample Preparation​Instrument​SP-NE32 Automated Nucleic Acid Extractor​950-000020-00​
Reagents​MGIEasy Nucleic Acid Extraction Kit​1000023774 (32 RXN)​
MGIEasy Microbiome DNA Extraction Kit​1000027955 (96 RXN)​
MGIEasy Stool Microbiome DNA Extraction Kit II​940-001247-00 (96 RXN)​
Library Preparation​Instrument​SP-100 Automated Sample Preparation System​900-000206-00​
Reagents​DNBSEQ Fast FS Library Prep Kit V2.0​940-001511-00 (16 RXN)​
940-001509-00 (96 RXN)​
DNBSEQ OneStep Make DNB Reagent Kit V4.0​940-001750-00 (Dual Barcode, 4RXN)​
Sequencing​Instrument​DNBSEQ-G99 Genetic Sequencer​900-000713-00​
Reagents​FCL SE100/PE50 High-Throughput Sequencing Set​940-000904-00​
FCL PE150 High-Throughput Sequencing Set​940-000905-00​
Analysis​Software​PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution)​900-000399-00​
Workflow ​Type​Product name​Catalog No.​
Sample Preparation​Instrument​STP-7000 High-Throughput Automated Sample Transfer System​900-000336-00​
SP-960 High-Throughput Automated Sample Preparation System (Config. 9)​900-000154-00​
Reagents​MGIEasy Stool Microbiome Extraction Kit II​940-001247-00 (96 RXN)​
940-001246-00 (384 RXN)​
Library Preparation​Instrument​SP-960 High-Throughput Automated Sample Preparation System (Config. 7)900-000152-00​
Reagents​DNBSEQ Fast FS Library Prep V2.0 Kit​940-001509-00 (96RXN)​
QC / Normalization / Pooling​Instrument​SP-Smart 8 Automated Liquid Handling System (Config. 4)900-000495-00​
Sequencing​Instrument​DNBSEQ-G400 Genetic Sequencer​900-000641-00​
Reagents​FCL PE150 High-Throughput Sequencing Set​940-000810-00​
Analysis​Hardware​ZTRON Lite Data Center​900-000673-00​
Software​PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution)​900-000399-00​
Workflow ​Type​Product name​Catalog No.​
Sample Preparation​Instrument​STP-7000 High-Throughput Automated Sample Transfer System​900-000336-00​
SP-960 High-Throughput Automated Sample Preparation System (Config. 9)​900-000154-00​
Reagents​MGIEasy Stool Microbiome Extraction Kit II​940-001247-00 (96 RXN)​
940-001246-00 (384 RXN)​
Library Preparation​Instrument​SP-960 High-Throughput Automated Sample Preparation System (Config. 7)​900-000152-00​
Reagents​DNBSEQ Fast FS Library Prep V2.0 Kit​940-001509-00 (96RXN)​
QC / Normalization / Pooling​Instrument​SP-Smart 8 Automated Liquid Handling System (Config. 4)​900-000495-00​
Sequencing​Instrument​DNBSEQ-T7 Genetic Sequencer​900-000698-00​
Reagents​FCL PE150 High-Throughput Sequencing Set​940-000836-00​
Analysis​Hardware​ZTRON Lite Data Center​900-000673-00​
Software​PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution)​900-000399-00​

3rd Party Kit Compatibility

DNBSEQ-E25 Sequencer

Sequencing Reagent​Catalog No. ​
App-C FCL SE100 High-throughput Sequencing Set​940-000895-00​
App-C FCL PE150 High-throughput Sequencing Set​940-000901-00​

DNBSEQ-G99 Sequencer

Library Conversion Kits​Catalog No.​
OneStep Library Conversion Kit V4.0​940-001648-00​
DNBSEQ Universal Library Conversion Kit​940-000963-00​

DNBSEQ-G400 Sequencer

You need to order only one of these kits.

Library Conversion Kits​Catalog No.​
OneStep Library Conversion Kit V4.0​940-001648-00​
DNBSEQ Universal Library Conversion Kit​940-000963-00​

You need to order both of the reagents.

Sequencing Reagent​Catalog No. ​
FCL PE150 High-throughput Sequencing Set​940-000810-00​
App-D PE Primer Kit​940-000917-00​

DNBSEQ-T7 Sequencer

You need to order only one of these kits.

Library Conversion Kits​Catalog No.​
OneStep Library Conversion Kit V4.0940-001648-00​
DNBSEQ Universal Library Conversion Kit​940-000963-00​

You need to order both of the reagents.

Sequencing Reagent​Catalog No. ​
FCL PE150 High-throughput Sequencing Set​940-000836-00​
App-D PE Primer Kit​940-000917-00​