DNBSEQ Fast PCR-FREE FS Library Prep Set

The DNBSEQ Fast PCR-FREE FS Library Kit is designed for preparing Whole Genome Sequencing (WGS) PCR-Free libraries compatible with Complete Genomics DNBSEQ sequencers. This kit streamlines the library preparation process by combining fragmentation, end-repair, A-tailing and cleanup into a single step, significantly reducing overall time while ensuring high-quality results. Compatible with a wide DNA input range (25 – 900 ng) and multiple sample types for ultimate flexibility.

This kit provides increased compatibility across a wide range of applications: WGS (low pass, human/animal/plant (30x), microbial) and virus long amplicon sequencing.

Libraries generated with this kit can be sequenced directly on the DNBSEQ-T1+ and DNBSEQ-T7+ platforms. For sequencing on the DNBSEQ-G99 or DNBSEQ-T7, either the Dual Barcode Circularization Kit or the DNBSEQ OneStep Make DNB Reagent Kit V4.0 is required.

Specifications

Product Name

DNBSEQ Fast PCR-FREE FS Library Prep Set V2.0

RXNs

16 RXN, 96 RXN, 384 RXN

Sample Type

gDNA from blood, saliva, oral swabs, tissue, microbial gDNA, metagenomic DNA

Input Quantity

25 ~ 900 ng

Strand Specificity

Single-sided bead purification: 600 – 850 bp
Dual-sided bead purification: 400 – 600 bp

Barcode

384 Single Barcodes

Assay Time

~1.5 hours

Hands-On Time

~20 minutes

Automated Library Prep

SP-100 (16 RXN)
SP-960 (96 RXN)

Read Length

PE100
PE150

Sequencing Platforms

DNBSEQ-G99DNBSEQ-T1+DNBSEQ-T7, DNBSEQ-T7+

Species Category

Human, animal, plant, microbe

Applications

Human WGS, plant & animal WGS, low-pass WGS, microbial WGS, metagenomic sequencing, virus long amplicons sequencing

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