DNBSEQ™ Fast RNA Library Prep Set

The DNBSEQ Fast RNA Library Kit is designed for preparing RNA libraries for the Complete Genomics DNBSEQ sequencers. Compatible with a wide RNA input range (10 ng – 1 ug) and multiple sample types for ultimate flexibility.

This automation-friendly kit streamlines the library preparation process by combining cDNA second strand synthesis and end-repair steps, significantly reducing overall time (~4 hrs) while ensuring high-quality results. This kit also includes additional flexibility in the second strand synthesis step. It comes with two buffer options (dNTP buffer and dUTP buffer) allowing users the option to either prepare a directional or non-directional library.

Libraries generated with this kit can be sequenced directly on the DNBSEQ-T1+ and DNBSEQ-T7+ platforms. For sequencing on the DNBSEQ-G99 or DNBSEQ-T7, either the Dual Barcode Circularization Kit or the DNBSEQ OneStep Make DNB Reagent Kit V4.0 is required.

Specifications

Product Name

DNBSEQ Fast RNA Library Prep Set

RXNs

16 RXN, 96 RXN, 192 RXN

Sample Type

Total (bulk) RNA from blood, saliva, fresh tissue, FFPE RNA, plant, animal, microbial, and meta

Input Quantity

10 ng ~ 1 ug total RN

Strand Specificity

Non-stranded or stranded library prep

Note: Kit comes with two buffers (dNTP buffer, dUTP buffer) to allow users to select the type of library preparation method

Barcode

288 Unique Dual Barcodes

Assay Time

~4.0 hours

Hands-On Time

~1 hour

Automated Library Prep

SP-100 (16 RXN)
SP-Smart8
SP-960 (96 RXN, 192 RXN)

Read Length

PE100
PE150

Sequencing Platforms

DNBSEQ-G99DNBSEQ-T1+DNBSEQ-T7, DNBSEQ-T7+

Species Category

Human, animal, plant, microbe

Applications

Whole-Transcriptome Sequencing, mRNA sequencing, long non-coding RNA sequencing, RNA-Seq, Meta transcriptome sequencing

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