DNBSEQ Fast FS Library Prep Set

The DNBSEQ Fast FS Library Kit is designed for preparing DNA libraries compatible with Complete Genomics DNBSEQ sequencers. This automation-friendly kit streamlines the library preparation process by combining fragmentation, end-repair, A-tailing and cleanup into a single step, significantly reducing overall time (~2 hrs) while ensuring high-quality results. Compatible with a wide DNA input range (1 – 1,000 ng) and multiple sample types for ultimate flexibility.

This kit provides increased compatibility across a wide range of applications: WGS (human/animal/plant (30x), microbial), WES, amplicon sequencing, and metagenomic sequencing.

Libraries generated with this kit can be sequenced directly on the DNBSEQ-T1+ and DNBSEQ-T7+ platforms. For sequencing on the DNBSEQ-G99 or DNBSEQ-T7, either the Dual Barcode Circularization Kit or the DNBSEQ OneStep Make DNB Reagent Kit V4.0 is required.

Specifications

Product Name

DNBSEQ Fast FS Library Prep Set V2.0

RXNs

16 RXN, 96 RXN, 192 RXN

Sample Type

gDNA from blood, saliva, oral swabs, tissue, microbial gDNA, metagenomic DNA, plants

Input Quantity

1 ~ 1,000 ng

Strand Specificity

Single-sided bead purification: 300 – 2,000 bp
Dual-sided bead purification: 450 – 550 bp

Barcode

288 Unique Dual Barcodes

Assay Time

~2.0 hours

Hands-On Time

~20 minutes

Automated Library Prep

SP-100 (16 RXN)
Smart 8 (48 RXN)
SP-960 (96 RXN)

Read Length

PE100
PE150

Sequencing Platforms

DNBSEQ-G99DNBSEQ-T1+DNBSEQ-T7, DNBSEQ-T7+

Species Category

Human, animal, plant, microbe

Applications

Human WGS, plant & animal WGS, microbial WGS, WES, metagenomic sequencing, amplicon sequencing

A Complete Solution for Every Step of Your Workflow