DNBSEQ-T7

This study demonstrates incorporation of multi-omics analyses with Stereo-Seq results to achieve unmatched characterization of the complex tumor immune microenvironment. Corroboration of spatial gene expression with previous findings, as well as strong similarity between sections within a sample demonstrate accuracy and precision of results. Novel marker genes within tumor subtypes may reveal new therapeutic targets.

This study demonstrates that Olink Explore HT is highly compatible with the DNBSEQ-T7 Genetic Sequencer, and the resulting data is comparable with Illumina NextSeq. The libraries generated from the Explore HT kit were first circularized by the MGIEasy Olink Circularization Set to enable compatibility with the DNBSEQ sequencing platforms. The circularized libraries were then sequenced on the DNBSEQ-T7 with App-D sequencing primer spiked into the PE100 sequencing cartridge. Comparative results between Complete Genomics actual data and Illumina NextSeq demonstrate that the Olink Explore HT kit is perfectly compatible with the DNBSEQ-T7.