DNBSEQ-G400

The successful integration of the SEQuoia RNA library preparation workflow with the DNBSEQ-G400
sequencing platform not only enhances accessibility to RNA sequencing but also presents a high quality and cost-effective
alternative to conventional methods. The SEQuoia RNA-Seq libraries exhibit excellent Q30 scores and high-quality reads on the DNBSEQ-G400 sequencing platform.

Generating DNA nanoballs(DNBs) prior to loading into flow cells prevents optical duplicates or ExAmp duplicates observed in other sequencing platforms
utilizing cluster generation.

The study compared Complete Genomics DNBSEQ-G400 and Illumina NovaSeq 6000 on single-RNA libraries from Vevo Therapeutics’ cancer cell lines, prepared using Parse Biosciences kits. DNBSEQ-G400 showed comparable or better single-cell sequencing results than NovaSeq 6000.

NGS technology allows for cost-effective, high-resolution detection of genetic variations related to diseases. Essential for research and genetic services, it balances quality and costs using methods like whole genome sequencing (WGS), whole exome sequencing (WES), and targeted panels.