COOLMPS 600 SEQUENCER
Reveal the Full Spectrum of Genomics
From Complete WGS to Transcriptomes
The CoolMPS 600 Sequencer is a high-efficiency, mid-throughput benchtop sequencer designed for exceptional accuracy and extended read lengths. Powered by CoolMPS™ technology, it delivers Q40 accuracy, single-end reads of 600 bp or longer, and up to 2.8 Tb of data per run. Contiguous 600-base reads (SE600) improve mapping across highly variable and repetitive genomic regions, helping resolve complex variation and detect structural variants that shorter reads may miss.
CoolMPS 600 is ideal for a range of applications, including:
Q40 Accuracy
High-confidence sequencing data.
Longer Read Length
Resolve complex genomic and transcriptomic regions with SE 600.
Comprehensive
Run 2 flow cells independently at any time.
TECHNOLOGY
CoolMPS™ Technology
Higher Signal Intensity
CoolMPS™ technology utilizes nucleotides with natural bases and extension blocks, combined with labeled antibodies that are both base-specific and block-dependent.
Longer Read Length
In the traditional method, scarring—one of the main limiting factors for long-read lengths—can occur, but this issue is eliminated with the CoolMPS approach.
Higher Accuracy
Each nucleotide (dNTP) is linked to 2-4 fluorescent dyes via antibodies, resulting in higher signal intensity and much better imaging. This contributes to exceptional sequencing accuracy and reliable long-read performance.
HOW IT WORKS
Dual Flow Cell Operation
DNBSEQ CoolMPS 600 offers independent dual flow cell operation, allowing researchers to run two flow cells independently, at any time, with various sequencing options.
PUBLICATIONS
Powered by BiozSPECIFICATIONS
Performance Parameters
| # of Flow Cells | Readsᵃ | Read Length | Data Outputᵇ | Run Timeᶜ | Q40 |
|---|---|---|---|---|---|
| 2 | 2.4B | SE600 | 1.4 Tb | <50 hr | >85% |
| PE150 | 720 Gb | 27 hr | >90% |
a. Reads per FC. The maximum number of effective reads is based on the sequencing of an internal standard library. Actual output may vary with sample types and library preparation methods.
b. The listed data output is per flow cell, meaning the total output per run doubles when utilizing both flow cells.
c. Run time is based on the dual flow cell module, including sample loading, sequencing, and FASTQ generation.
Resolving Complex Genomic Regions with SE600 Reads
CoolMPS 600 delivers contiguous 600 base reads (SE600) to improve mapping and variant detection in challenging, repetitive regions of the genome. In HG002, SE600 reads revealed a 172-base-pair deletion in IL1RN that was missed in the PE150 dataset, demonstrating the value of longer reads for resolving complex variation. At 40× coverage, SE600 whole-genome sequencing achieved SNP and indel F1 scores of 0.998 and 0.991, respectively, against the T2T-based NIST v5.0 truth set, supporting accurate and comprehensive genome analysis.
Figure 1. SE600 reads improve mapping in repetitive regions compared with PE150 reads. The IL1RN gene (interleukin-1 receptor antagonist) contains an intronic region of 86-base-pair repeats that affects how the body regulates inflammatory signaling pathways. In the top panel, a heterozygous deletion of two copies (172 base pairs) is clearly seen in the SE600 data. The PE150 data in the bottom panel completely miss this deletion.
RESOURCES
Everything you need to know about CoolMPS 600
Resources
Ordering Information
| Category | Product | Cat. No. |
|---|---|---|
| Instrument | CoolMPS 600 Genetic Sequencer | 900-001240-00 |
| Sequencing sets | CoolMPS 600 Sequencing Set PE150 | 940-004002-00 |
| CoolMPS 600 Sequencing Set SE600 | 940-004001-00 |