Complete Genomics does not concentrate or dilute samples that are outside of the required concentration range, as described in the Sample Submission Instructions. The liquid handling systems used for sample and library preparation have not been validated for concentrations and volumes outside of the required ranges. Because samples cannot be accepted outside of the concentration range, and there can be variability between customer measurements and Complete Genomics measurements, we recommend that the sample have a concentration well within the range required, rather than at the border of the range.
If the DNA is too concentrated, it can be diluted by adding 1x TE, pH 8.0.
If the DNA is too dilute, recommended options to concentrate the sample include the following:
Note that there can be loss of DNA during sample concentration, as the yields are not 100%. For example, the estimated yield for genomic DNA using the QIAamp DNA Micro Kit is 60%. Using a speed vacuum for concentrating samples is not recommended due to the risk of cross-contamination and increasing the salt in the sample and should only be used when 100% yield is absolutely necessary. If taking this approach, take extra care to ensure that the sample is not completely dried, which can also result in reduced yield.