At this time, Complete Genomics accepts human DNA only. DNA may be extracted from blood, frozen tissue, cell lines, or saliva. Complete Genomics cannot accept DNA that has been treated with whole genome amplification (WGA) or that is derived from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA sample requirements are as follows:
|DNA amount, as measured by PicoGreen||Required: ≥ 10 μg
Recommended: ≥ 5 μg
|DNA concentration, as measured by PicoGreen
||75 to 300 ng/μl|
|DNA volume||50 to 200 μl|
|DNA fragment length||High molecular weight double-stranded genomic DNA,
majority over 20 kb
1x TE, pH 8.0
A260/280 ratios should be between 1.8 and 2.0. Values outside of this range suggest that there are impurities in the sample that could affect sequencing performance.
Note that sample acceptance is based on quantity measurements performed by Complete Genomics at the time of sample QC. Because there is inherent variability in quantitation between users and sites, Complete strongly encourages customers to submit as much DNA as possible to avoid delays in sample processing due to failure to meet sample acceptance criteria.